泰国淡水鱼虹彩病毒病原的检测与鉴定
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摘要
2003年4月至2004年3月期间,在泰国进行了虹彩病毒或兰氏病毒调查。对566例患病和健康的鱼和青蛙标本进行了检测,以确定其是否可能感染ranavirus或带菌。这些动物来自渔农自然护理署辖下的渔场、鱼市及水产动物诊所。共制备393份组织提取物用于病毒分离,393份DNA提取物用于ranavirus PCR检测。用EPC细胞系仅分离出两种病毒。从一种病鱼中分离到1株病毒,该病毒在个体养鱼场隔离期间,背部肌肉呈白色,死亡率超过50%。另一株病毒分离物从患病蛙蛙中获得。这些蛙蛙来自泰国东部斯拉基省的一个私人养鱼场。患病青蛙最初从柬埔寨进口,在讲台和腿上显示溃疡或伤口,死亡率超过50%。鬼鱼分离出的病毒具有DNA型基因组和包膜病毒颗粒。该鬼鱼病毒不属于ranavirus,因为特异性PCR检测结果为阴性。 The viral isolate from the frogs also has DNA type genome and enveloped virus particles. This frog virus gave a positive result in PCR detection indicating, it is a ranavirus. Molecular characterization and comparison showed that this new ranavirus isolate is closely related to Rana tigrina ranavirus, ranavirus AV9803 and frog virus 3 with 99%, 98% and 98% nucleotide homology. However, based on the evolutionary tree, the ranavirus from the diseased frogs, which was originally imported from Cambodia, is a new strain or variant of the genus Ranavirus. This new strain of ranavirus is different to the previous isolates of ranavirus obtained in Thailand. Therefore, movement and control measures for frogs and fish should be in place to prevent the invasion of new ranavirus strain into Thailand and into the region.
建议引用
Kanchanakhan, S, Polchana, J., & Nuanchan, T.(2005)。泰国淡水鱼虹彩病毒病原的检测与鉴定。K. Nagasawa(编),《东南亚鱼虾病诊断和预防的最新进展》(第95-104页)。菲律宾,伊洛伊洛:水产养殖部,东南亚渔业发展中心。
类型
本章国际标准图书编号
9718511732集合
- 东南亚鱼虾病诊断与预防的最新进展[43]