缺氧对斑节对虾类胡萝卜素代谢影响的初步研究
- 全球风格
- 美国心理学协会
- 爱思唯尔——哈佛大学
- 温哥华
- MLA
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摘要
黑虎虾Penaeodon的色素沉着是由于虾青素,虾青素单酯和虾青素二酯。这些类胡萝卜素是从β-胡萝卜素或玉米蛋白生物合成的。将生物合成假设以由酶C3和C4单氧化酶和Caroten-4-醇脱氢酶介导的。Blue-Shrimp综合征,其特征在于虾表皮的低总虾青素水平,与类胡萝卜素的营养缺乏有关,但也可以涉及高有机物,缺氧,高密度和高pH等因素。在这项研究中,研究了类胡萝卜素的宿主蛋白酶酸甲肽代谢对胡萝卜素的影响以及酶C3和C4单氧化酶和Caroten-4-Ol脱氢酶的活性研究了缺氧的影响。每种含有25次虾的碎片喂养治疗饮食(不含任何类胡萝卜素)两周。在接下来的两周内给予一个罐中的虾(治疗1)在接下来的两周内给予β-胡萝卜素(3.8 ppm),而另一个(治疗2)的那些(治疗2)被喂养治疗饮食。在引入试验饮食后两天,对两种治疗进行缺氧攻击(溶解氧,2.5-2.7mg / L)和所监测的粪便的类胡萝卜素含量和型材概况。在缺氧攻击后五天进行酶活性的测定。结果表明,治疗2(122.2ppm)的虾虾氏粪便总类胡萝卜素含量增加了10倍,与治疗1(12.4ppm)对缺氧攻击并在三天后恢复到基础水平(3.9-4.1ppm)。 The carotenoids found in the feces of shrimps in treatment 2 were astaxanthin, astaxanthin monoester, astaxanthin diesters, and an unidentified carotenoid which was also found in the muscle of stressed shrimp. Using beta-carotene as substrate, conversion to astaxanthin was not observed in the hepatopancreas homogenates of shrimps in both treatments. Instead, a slow conversion of astaxanthin to betacarotene (i.e., a reverse reaction) was noted in treatment 2 exhibiting twice the activity in treatment 1. Radiolabelling studies using 14C-labelled astaxanthin as substrate confirmed the existence of this enzyme-mediated reductive pathway from astaxanthin which occurs at a very slow rate. This study demonstrated the carotenoid-depleting effect of hypoxia on shrimp, both through enhanced fecal release and moderately elevated reductive pathway from astaxanthin. This effect suggests a possible mechanism by which blue-shrimp syndrome may develop.
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